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Seven commercial soybean paste were tested, for ACE inhibition effect. In purification of ACE inhibitor from No. 2 soybean paste, acetone fraction (50∼80%) had 57% protein yield with 92.8% ACE inhibition effect. Inhibitor was purified from acetone fraction of soybean paste by Sephadex G-25, Sephadex LH-20 and ODS column chromatography and HPLC. IC_(50) value of the purified inhibitor was 0.6 ㎎/㎖. The inhibitor showed the competitive inhibition patterns on ACE. Amino acid analysis showed that the peptides consist of Ala, Phe, Leu, Glu, Gly, Ser, and Asp.
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